primary atf4 mouse mab (Proteintech)
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Structured Review
Proteintech
primary atf4 mouse mab
Primary Atf4 Mouse Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+atf4+mouse+mab/ATF4+Antibody/pmc11776876-49-5-10
Average 96 stars, based on 619 article reviews
Primary Atf4 Mouse Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+atf4+mouse+mab/ATF4+Antibody/pmc11776876-49-5-10
Average 96 stars, based on 619 article reviews
primary atf4 mouse mab - by Bioz Stars,
2026-08
96/100 stars
Images
1) Product Images from "TARGETING S100A9-TLR2 AXIS CONTROLS MACROPHAGE NLRP3 INFLAMMASOME ACTIVATION IN FATTY LIVER ISCHEMIA REPERFUSION INJURY"
Article Title: TARGETING S100A9-TLR2 AXIS CONTROLS MACROPHAGE NLRP3 INFLAMMASOME ACTIVATION IN FATTY LIVER ISCHEMIA REPERFUSION INJURY
Journal: Shock (Augusta, Ga.)
doi: 10.1097/SHK.0000000000002470
Figure Legend Snippet: IR-induced injury leads to increased S100A9 and related pathway activation in fatty livers HFD fed C57 mice were used to establish liver IR models. Samples were harvested after 90-min ischemia and 6 h of reperfusion. (A) Serum ALT levels. (B) Immunofluorescence staining and quantification of CD11b + macrophages in ischemia fatty livers, scale bars: 50 μm, 20 μm. (C) Volcano Plot displaying 748 genes to be upregulated and 625 genes downregulated. (D) KEGG pathway enrichment analysis of major biological pathways contributing to fatty liver IR stress. (E) Heatmap showing the expression of different genes. (F) Western-assisted analysis and relative density ratio of S100A9, TLR2, and ATF4 in liver tissues. (G) Immunochemistry staining and quantification of S100A9 positive cells in ischemia fatty livers, scale bars: 50 μm, 20 μm. (H) Detection of cytokines IL-1β, CXCL1, CXCL-2, and IL-6 by qRT-PCR in the livers. N = 3–6/group. All data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001. ALT, alanine aminotransferase; HFD, high-fat diet; KEGG, Kyoto Encyclopedia of Genes and Genomes; IR, ischemia reperfusion.
Techniques Used: Activation Assay, Immunofluorescence, Staining, Expressing, Western Blot, Quantitative RT-PCR
Figure Legend Snippet: S100A9 induces inflammatory injury via macrophage TLR2 activation BMMs were isolated from WT mice and incubated with rS100A9 or rS100A8 respectively for 24 h. (A) Western-assisted analysis of TLR2 and ATF4 in rS100A9-stressed macrophages. (B) Western-assisted analysis of TLR2 and ATF4 in rS100A8-stressed macrophages; BMMs were transfected with the TLR2-siRNA or control vector followed by incubation with rS100A9. (C) Detection of cytokines IL-1β, CXCL1, CXCL-2, and IL-6 by qRT-PCR in BMMs. (D) Western-assisted analysis of ATF4 in nuclear extracts. (E) Immunofluorescence staining for ATF4 distribution in macrophages. DAPI was used to visualize nuclei. Scale bars: 50 μm, 20 μm. N = 3–6/group. All data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001. BBM, bone-derived macrophage. WT, wild type; qRT-PCR, quantitative real-time PCR.
Techniques Used: Activation Assay, Isolation, Incubation, Western Blot, Transfection, Control, Plasmid Preparation, Quantitative RT-PCR, Immunofluorescence, Staining, Derivative Assay, Real-time Polymerase Chain Reaction
Figure Legend Snippet: S100A9 knockdown attenuates liver dysfunction and inflammatory damage following IR of fatty livers C57 mice were injected with AAV9-S100A9-shRNA 48 h before establishing liver IR models using HFD-fed mice. Samples were harvested after 90-min ischemia and 6 h of reperfusion. (A) Western-assisted analysis and relative density ratio of S100A9 in liver tissues. (B) H&E staining of ischemic fatty livers, scale bar: 200 μm, 100 μm. (C) Serum ALT levels. (D) Immunofluorescence staining and quantification of CD11b positive macrophages and immunochemistry staining and quantification of Ly6G positive cells in ischemia fatty livers, scale bar: 40 μm. (E) Western-assisted analysis of TLR2 and ATF4 in liver tissues. (F) Dual immunofluoresence staining for CD68 (red) and TLR2 (green) co-localization in injured fatty livers after IR stimulation, scale bars: 50 μm, 20 μm. (G) Detection of cytokines IL-1β, CXCL1, CXCL-2, and IL-6 by qRT-PCR in the livers. N = 3–6/group. All data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001. HFD, high-fat diet; IR, ischemia reperfusion. qRT-PCR, quantitative real-time PCR.
Techniques Used: Knockdown, Injection, shRNA, Western Blot, Staining, Immunofluorescence, Quantitative RT-PCR, Real-time Polymerase Chain Reaction
Figure Legend Snippet: ATF4 is responsible for regulating S100A9-triggered NEK7/NLRP3 inflammasome activation in macrophages BMMs were transfected with the ATF4-siRNA or control vector followed by incubation with rS100A9 . (A) Detection of cytokines IL-1β, CXCL1, CXCL-2, and IL-6 by qRT-PCR in BMMs. (B) IL-1β release from BMMs. (C) Western-assisted analysis of NEK7 and NLRP3 in BMMs. (D) Dual immunofluoresence staining for NEK7 (red) and NLRP3 (green) co-localization in BMMs, scale bars: 40 μm, 20 μm. N = 3–6/group. All data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001. BBM, bone-derived macrophage.
Techniques Used: Activation Assay, Transfection, Control, Plasmid Preparation, Incubation, Quantitative RT-PCR, Western Blot, Staining, Derivative Assay
Figure Legend Snippet: The schematic diagram of S100A9 regulating IR-induced macrophage NLRP3 inflammasome activation in fatty livers. During fatty liver IR stress, S100A9 is released and accumulated in injured livers. S100A9 interacts with macrophage TLR2, promoting ATF4 activation and the formation of NEK7/NLRP3 inflammasome. It leads to subsequent inflammatory response and liver damage. IR, ischemia reperfusion.
Techniques Used: Activation Assay
